Genomic Labeling Kits - Details & Specifications

SureTag Complete DNA Labeling Kit
The SureTag Complete DNA Labeling Kit provides all the necessary reagents for a complete enzymatic labeling solution. All components for sample fragmentation, labeling and clean-up are included. Two vials of Agilent Male and Female Human Reference DNA are also included. The reference DNA is a high quality, genotyped DNA from normal male and female individuals, Caucasian ethnicity. The SureTag Complete DNA Labeling Kit generates robust cyanine-labeled genomic DNA targets for optimal hybridization signals with high signal-to-noise ratio and low probe-to-probe variability. Efficient labeling provides reproducibility, confidence and maximum sensitivity in your experimental results. Superior results and complete workflow for Agilent oligonucleotide array-based CGH, CGH+SNP, CNV, ChIP-on-chip and DNA Methylation workflows The kit is manufactured under ISO 13485 compliance and validated for use with Agilent microarrays Sufficient reagents for 50 labeling reactions (or 100 reactions for the 8-pack) Sample required per reaction is 500 ng genomic DNA or 3 µg amplified genomic DNA DNA columns are included for the removal of the unincorporated dye after labeling
Kit Contents	Male and Female Human Reference DNA, 25 µg each Restriction digest enzyme Alu I, 25 µL Restriction digest enzyme Rsa I, 25 µL Buffer C, 130 µL BSA, 10 µL Random Primers, 265 µL Exo-Klenow, 55 µL Cyanine 3-dUTP, 75 µL Cyanine 5-dUTP, 75 µL 10X dNTP Mix, 265 µL 5X Reaction Buffer, 525 µL Nuclease-free water, 1.5 mL Purification Columns, 50 units
Storage Conditions	Male and female reference DNA should be stored at +4°C; clean up columns should be stored at room temperature; Cyanine 3- and 5-dUTP and remaining kit components should be stored at -20°C.

SureTag DNA Labeling Kit
The SureTag DNA Labeling Kit provides all the reagents for sample fragmentation, enzymatic labeling and clean-up. The SureTag DNA Labeling Kit generates robust cyanine-labeled genomic DNA targets for optimal hybridization signals with High Signal to Noise ratio and Low Probe to Probe Variability. Efficient labeling provides reproducibility, confidence and maximum sensitivity in your experimental results. Agilent recommends using SureTag Complete DNA Labeling kit that also includes Genomic Reference DNA for a complete solution. Superior results and complete workflow for Agilent oligonucleotide array-based CGH, CGH+SNP, CNV, ChIP-on-chip and DNA methylation workflows The kit is manufactured under ISO 13485 compliance and validated for use with Agilent microarrays Sufficient reagents for 50 labeling reactions (or 100 reactions for the 8-pack) Sample required per reaction is 500 ng genomic DNA or 3 µg amplified genomic DNA This kit does not contain Reference DNA
Kit Contents	Restriction digest enzyme Alu I, 25 µL Restriction digest enzyme Rsa I, 25 µL Buffer C, 130 µL BSA, 10 µL Random Primers, 265 µL Exo-Klenow, 55 µL Cyanine 3-dUTP, 75 µL Cyanine 5-dUTP, 75 µL 10X dNTP Mix, 265 µL 5X Reaction Buffer, 525 µL Nuclease-free water, 1.5 mL Purification Columns, 50 units
Storage Conditions	Clean up columns should be stored at room temperature; Cyanine 3- and 5-dUTP and remaining kit components should be stored at -20°C.

Genomic DNA ULS Labeling Kit

Utilizing Kreatech's Universal Linkage System (ULS™), Agilent's Genomic DNA ULS Labeling Kit efficiently generates Cy-labeled genomic targets for use with the Agilent Oligo aCGH system. Effective for genomic DNA isolated from tissues, cells, blood and FFPE samples, this non-enzymatic method directly labels genomic DNA with fluorescent dyes, thereby avoiding the typical challenges you face with degraded samples obtained from FFPE tissues.

Superior results and complete workflow for Agilent oligonucleotide array-based CGH, CNV, workflows
The kit is manufactured under ISO 13485 compliance and validated for use with Agilent microarrays
This kit does not contain Reference DNA

Kit Contents

Reagents (4 vials):
ULS™-Cy™ 3 Reagent (12.5 µL)
ULS™-Cy™ 5 Reagent (12.5 µL)
10X Labeling Solution (100 µL)
Agilent-CGHblock (750 µL)

Columns (2 pkgs):
Agilent-KREApure™ purification column, 1 pk/12
Collection tubes, 1 pk/12

Genomic DNA High-Throughput ULS Labeling Kit

Sufficient two-color labeling reaction reagents for 16 samples (1-pack format), 24 samples (2-pack format), 48 samples (4-pack format) and 96 samples (8-pack format)
Input requirement is 0.25 - 2 ug per reaction depending on the array format of choice and sample type
Utilizes a non-enzymatic, direct labeling technology without the need of template amplification
Agilent-CGHblock should be stored at -20°C; remaining kit contents should be stored at 4°C

Kit Contents

Reagents (4 vials):
ULS™-Cy™ 3 Reagent (30 µL)
ULS™-Cy™ 5 Reagent (30 µL)
10X Labeling Solution (120 µL)
Agilent-CGHblock (2.6 mL)

For Research Use Only. Not for Use in Diagnostic Procedures