DNA Analysis ScreenTape - Details & Specifications

DNA analysis in the NGS workflow

The 4200 TapeStation system can be used at several quality control points in the NGS workflow: genomic DNA QC, shearing of genomic DNA, library amplification, and post-capture amplified library after SureSelect targeted enrichment. This figure shows post-sheared genomic DNA analyzed on the D5000 ScreenTape assay in different concentrations.

 

Analytical specifications D1000 ScreenTape High Sensitivity D1000
ScreenTape
D5000 ScreenTape High Sensitivity D5000
ScreenTape
Sizing range 35 – 1000 bp 35 – 1000 bp 100 bp – 5,000 bp 100 bp – 5,000 bp
Typical Resolution 35-300 bp: 15 % 35-300 bp: 15 % 400-5000bp: 15% 400-5000bp: 15%
  300-1000 bp: 10 % 300-1000 bp: 10 %    
Sensitivity 1 0.1 ng/µL 5 pg/µL 0.1ng/µl 5pg/µl
Sizing precision 2  5% CV 5% CV 5% CV 10% CV
Sizing accuracy  ± 10 % 3 ± 10 % 3 ±10% ±15%
Quantification precision 0.1 – 1ng/µL 15% CV 15 % CV 0.1-1ng/µl  15%CV 15% CV
  1 – 50ng/µL 10% CV   1-50ng/ul  10% CV  
Quantitative accuracy 4 ± 20 % ± 20 % ±20% ±25%
Qualitative range 0.1 – 50 ng/µL 10 - 1000 pg/µL 0.1-50ng/µl 10-1000pg/µl
Maximum sample buffer strength 20 mM KCl
60 mM Phosphate Buffer
60 mM Guanidine-HCl
240 mM NaCl
60 mM Acetate
7 mM KCL
20 mM Phosphate Buffer
20 mM Guanidine-HCl
80 mM NaCl
20 mM Acetate
250mM KCl
250mM Tris-HCl
25mM Guanidine-HCl
125mM NaCl
50mM Acetate
25mM MgCl2
25mM BSA

25mM KCl
25mM Tris-HCl
2.5mM Guanidine-HCl
12.5mM NaCl
5mM Acetate
2.5mM MgCl2
2.5mM BSA

Physical specifications
Analysis time 16 samples < 20 min 16 samples < 20 min 15 samples < 25 min 15 samples < 20 min
  96 samples < 100 min 96 samples < 100 min 96 samples < 135 min 96 samples < 120 min
Samples per consumable 16 16 15 15
Sample volume required 1 μL 2 µL 1 µL 2 µL
Kit stability 4 months 4 months 4 months 4 months
Kit size 112 samples 112 samples 105 samples 105 samples

1 Signal/noise ratio >3 for a single peak
2 Determined using the respective ladder as sample
3 Accuracy for software ladder: ± 20 %
4 Measured against 2200 TapeStation system

For Research Use Only. Not for use in diagnostic procedures