DNA Analysis ScreenTape - Details & Specifications

DNA analysis in the NGS workflow

The 4200 TapeStation system can be used at several quality control points in the NGS workflow: genomic DNA QC, shearing of genomic DNA, library amplification, and post-capture amplified library after SureSelect targeted enrichment. This figure shows post-sheared genomic DNA analyzed on the D5000 ScreenTape assay in different concentrations.

 

Analytical specifications D1000 ScreenTape High Sensitivity D1000
ScreenTape
D5000 ScreenTape High Sensitivity D5000
ScreenTape
Sizing range 35 - 1,000 bp 35 - 1,000 bp 100 - 5,000 bp 100 – 5,000 bp
Typical resolution 35 - 300 bp: 15% 35 - 300 bp: 15% 400 - 5,000 bp: 15% 400 - 5,000 bp: 15%
  300 - 1,000 bp: 10% 300 - 1,000 bp: 10%    
Sensitivity1 0.1 ng/µL 5 pg/µL 0.1 ng/µL 5 pg/µL
Sizing precision2  5% CV 5% CV 5% CV 10% CV
Sizing accuracy2 ±10%3 ±10%3 ±10% ±15%
Quantification precision 0.1 - 1 ng/µL:15% CV 15% CV 0.1 - 1 ng/µL:15% CV 15% CV
  1 - 50 ng/µL:10% CV   1 - 50 ng/µL:10% CV  
Quantitative accuracy4 ±20% ±20% ±20% ±25%
Qualitative range 0.1 - 50 ng/µL 10 - 1,000 pg/µL 0.1 - 50 ng/µL 10 - 1,000 pg/µL
Maximum sample buffer strength 20 mM KCl
60 mM Phosphate Buffer
60 mM Guanidine-HCl
240 mM NaCl
60 mM Acetate
7 mM KCl
20 mM Phosphate Buffer
20 mM Guanidine-HCl
80 mM NaCl
20 mM Acetate
250 mM KCl
250 mM Tris-HCl
25 mM Guanidine-HCl
125 mM NaCl
50 mM Acetate
25 mM MgCl2
25 mM BSA

25 mM KCl
25 mM Tris-HCl
2.5 mM Guanidine-HCl
12.5 mM NaCl
5 mM Acetate
2.5 mM MgCl2
2.5 mM BSA

Physical specifications
Analysis time 16 samples < 20 min 16 samples < 20 min 15 samples < 25 min 15 samples < 20 min
  96 samples < 90 min 96 samples < 105 min 96 samples < 135 min 96 samples < 120 min
Samples per device 16 16 15 15
Sample volume required 1 μL 2 µL 1 µL 2 µL
Kit stability 4 months 4 months 4 months 4 months
Kit size 112 samples 112 samples 105 samples 105 samples

1 Signal/noise ratio >3 for a single peak
2 Determined using the respective ladder as sample
3 Accuracy for electronic ladder: ±20%
4 Measured against 2200 TapeStation system

For Research Use Only. Not for use in diagnostic procedures