DNA Analysis Kits & Reagents - Details & Specifications

The DNA 1000, 7500 and 12000 kits offer automated analysis of PCR fragments, restriction digests and next-generation sequencing (NGS) samples. Typical application areas are: amplicon QC in forensic DNA studies, gene expression analysis by RT-PCR, DNA QC for NGS, or detection of genetically modified organisms.

The DNA1000 kit outperforms traditional slab gels when analyzing a mixture of 13 multiplex PCR products with its high resolution and the reliable sizing and quantitation of each individual DNA fragment. (Application note 5988-9342EN)

 

Analytical specifications DNA 1000 Kit DNA 7500 Kit DNA 12000 Kit
Sizing range 25–1000 bp 100–7500 bp 100–12000 bp
Sizing resolution 25–100 bp: 5 bp
100–500 bp: 5%
500–1000 bp: 10%
100–1000 bp: 5%
1000–7500 bp: 15%
100–1000 bp: 5%
1000–12000 bp: 15%
Sizing accuracy ± 10% ± 10% ± 15%
Sizing reproducibility 5% CV 5% CV 5% CV
Quantitation accuracy1 20% 20% 25%
Quantitation reproducibility1 25–500 bp:15% CV
500-1000 bp:5% CV
100-1000 bp:10% CV
1000-7500 bp: 5% CV
100-1000 bp: 15% CV
1000-12000 bp: 10% CV
Quantitative range1 0.5-50 ng/µL 0.5-50 ng/µL 0.5-50 ng/µL
Maximum salt concentration in sample 250 mM for KCl,
15 mM for MgCl2,
250 mM NaCl
250 mM for KCl,
15 mM for MgCl2,
250 mM NaCl
250 mM for KCl,
15 mM for MgCl2,
250 mM NaCl
Physical specifications
Analysis time 35 minutes 30 minutes 30 minutes
Samples per chip 12 12 12
Sample volume 1 µL 1 µL 1 µL
Kit stability 4 months (see box for storage temperatures) 4 months (see box for storage temperatures) 4 months (see box for storage temperatures)
Kit size 25 chips

12 samples/chip
= 300 samples/kit
25 chips

12 samples/chip
= 300 samples/kit
25 chips

12 samples/chip
= 300 samples/kit

 

1Determined using the respective DNA ladder as sample

For Research Use Only. Not for use in diagnostic procedures