RNA Kits & Reagents - Details & Specifications

RNA quality control and quantitation is essential before any downstream application. Both RNA kits offer:

  • Quantitation and integrity analysis of total RNA or mRNA
  • RNA Integrity Number (RIN) – to compare integrity of total RNA samples
  • Visualization of rRNA impurities in mRNA samples
  • High sensitivity RNA analysis, e.g. samples derived from microdissection



RNA QC
The electropherograms shows the analysis of high quality total RNA (RIN 9.2) in comparison to a partially degraded total RNA (RIN 5.8). (Application Note 5989-1165EN)

 

Analytical specifications RNA 6000 Nano Kit RNA 6000 Pico Kit
total RNA mRNA total RNA mRNA
Quantitative range 25 - 500 ng/µL 25 - 250 ng/µL - -
Qualititative range 5 - 500 ng/µL 5 - 250 ng/µL 50 - 5000 pg/µL in water 250 - 5000 pg/µL in water
Sensitivity (S/N>3) 5 ng/µL in water 25 ng/µL in water 50 pg/µL in water or
200 pg/µL in TE
250 pg/µL in water or
500 pg/µL in TE
Quantitation reproducibility
(within a chip)
10% CV 10% CV 20% CV 20% CV
Quantitation accuracy1 20% 20% 30% -
Maximum sample buffer strength 100 mM Tris
0.1 mM EDTA
or 125 mM NaCl
15 mM MgCl2
100 mM Tris
0.1 mM EDTA
or 125 mM NaCl
15 mM MgCl2
50 mM Tris
0.1 mM EDTA
or 50 mM NaCl
15 mM MgCl2
50 mM Tris
0.1 mM EDTA
or 50 mM NaCl
15 mM MgCl2
Physical specifications
Analysis time 30 minutes 30 minutes 30 minutes 30 minutes
Samples per chip 12 12 11 11
Sample volume 1 µL 1 µL 1 µL 1 µL
Kit stability =4 months at 4°C =4 months at 4°C =4 months at 4°C =4 months at 4°C
Kit size 25 chips

12 samples/chip
= 300 samples/kit
25 chips

12 samples/chip
= 300 samples/kit
25 chips

11 samples/chip
= 275 samples/kit
25 chips

11 samples/chip
= 275 samples/kit

1Determined analyzing the RNA ladder as sample

For Research Use Only. Not for use in diagnostic procedures