MxPro QPCR Software

The MxPro software is included with the purchase of the MxPro QPCR System. To activate the software, download key.


Software Features

  • Powerful, flexible, easy-to-use
  • Perform multiple sub-experiments on a single plate
  • Real-time amplification plots viewed and results analyzed during run
  • Standard curves plotted for multiple dyes on a single chart
  • Melting curve analysis with automatic melting temperature (Tm) calculation
  • Allelic discrimination by threshold cycle or by endpoint fluorescence values with auto-call feature
  • Export chart and text report data directly from display screen to Microsoft® Excel, PowerPoint, text and image fi
  • Software can link up to six (6) Mx3005P and Mx3000P instruments with one computer
  • View all dyes on one amplification plot, or view each single dye in multiplex experiments
  • View and analyze data in multiple formats including amplification plots, scatter plots, fluorescence intensity screens, final call results, melting curves,     annealing range, and text reports
  • All results available as raw data. Easily export data to Excel, PowerPoint and text files
  • Flexible analysis options: amplification plots, scatter plots, standard curve charts, fluorescence intensity, final call results, melting curve, annealing range and text report
  • View all dyes for multiplex experiments on one amplification plot and/or standard curve chart or view individual plots for each individual dye
  • Software compatible with Windows 7 (32-bit), XP, and Vista



Plate Setup allows you to import from previous experiment or templates, assign assay/gene target names to dyes, and auto increment replicates and standard dilutions. Add well name information to identify sample name, number, quantity, or any other specific sample information.

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Amplification plot and standard curve can be displayed simultaneously and changes in threshold are instantly displayed. In this figure, the "Adapative Baseline" algorithm ensures accurate quantification across five gene targets of varying abundance.


Perform automated gene expression analysis by the "Comparative Quantitation" module in the MxPro software. In this example, normalized relative quantity for six gene targets across two treatments is calculated and displayed on a log base 2 scale with upper and lower error limits based on replicate variability.