RNA Integrity Number (RIN)
The RNA integrity number (RIN) is a software tool designed to help scientists estimate the integrity of total RNA samples. The expert software automatically assigns an integrity number to an eukaryote total RNA sample.Using this tool, sample integrity is no longer determined by the ratio of the ribosomal bands, but by the entire electrophoretic trace of the RNA sample. This includes the presence or absence of degradation products. In this way, interpretation of an electropherogram is facilitated, comparison of samples is enabled and repeatability of experiments is ensured. The assigned RIN is independent of sample concentration, instrument and analyst therefore becoming a de facto standard for RNA integrity.
The RNA Integrity Database (RINdb) is a freely accessible repository holding hundreds of user submitted total RNA traces. By searching the database scientists can now see what is a "normal" profile for different tissue types as well as the effects of using different RNA extraction methods and kits.
What the RIN can do:
What the RIN cannot do:
- Schroeder A, Mueller O, Stocker S, Salowsky R, Leiber M, Gassmann M, Lightfoot S, Menzel W, Granzow M and Ragg T. The RIN: an RNA integrity number for assigning integrity values to RNA measurements.
- BMC Molecular Biology 2006, 7:3 (31 Jan 2006) Imbeaud S, Graudens E, Boulanger V, Barlet X, Zaborski P, Eveno E, Mueller O, Schroeder A, and Auffray C. Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces, Nucl. Acids Res. 2005 33: e56.
- Agilent Publication 5989-1165EN. RNA Integrity Number (RIN)- Standardization of RNA Quality Control