qPCR Tools and Links

Primer/Probe Design Software Tools Website Address
Primer3 http://primer3.sourceforge.net/
IDT PrimerQuest http://www.idtdna.com/Scitools/Applications/Primerquest/
Oligonucleotide Properties Calculators  
Zuker mfold http://mfold.bioinfo.rpi.edu/
IDT OligoAnalyzer http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/
RNAture http://www.rnature.com/oligonucleotide.html
Oligo Houses  
Biosearch Technologies http://www.biosearchtech.com/products/feature/order.asp
IDT Technologies http://www.idtdna.com/
Operon http://www.operon.com
Sigma-Aldrich http://www.sigmaaldrich.com/life-science/custom-oligos.html
TriLink Biotechnologies http://www.trilinkbiotech.com/products/oligo/oligo_synthesis.asp
Other Q- PCR Links  
Gene Quantification site http://www.gene-quantification.info/
Real-Time Primer Database http://medgen.ugent.be/rtprimerdb/
Real-Time PCR Primers http://www.realtimeprimers.org
Primer Bank (Harvard) http://pga.mgh.harvard.edu/primerbank/
geNORM (Reference gene normalization software) http://medgen.ugent.be/~jvdesomp/genorm/
RefSeq (Reference sequence database) http://www.ncbi.nih.gov/RefSeq/
Spidey (cDNA to genomic alignment tool) http://www.ncbi.nlm.nih.gov/spidey/
All about Molecular Beacons http://www.molecular-beacons.org/
General Molecular Biology Links and Tools http://iubio.bio.indiana.edu
TATAA Biocenter http://www.tataa.com
Discussion Groups  
realtimepcr From this web site, run a Search for “real time PCR ” for additional groups

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Agilent Technologies has no affiliation with any of the companies providing these applications.

For Research Use Only. Not for use in diagnostic procedures