Mx3005P QPCR Systems - Details & Specifications

The Mx3005P system is among the most reliable and trusted QPCR instruments available, with a long record of citation in peer-review journals. Offering unmatched flexibility and reliability, the systems are ideal for a wide variety of applications and chemistries (including but not limited to: gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis and chromatin immune-precipitation studies.

Highly reproducible results are the product of the Mx3005P’s single light source, single detector precision optic scanning design, providing uniform excitation and detection, coupled with the trusted Peltier-based thermal system, which ensures uniform ramping and thermal accuracy.

The advanced optical system used by the Mx3005P offers numerous benefits:

Eliminates optical variation by ensuring uniform excitation intensity and emission measurement from well-to-well, across the entire plate. Elimination of optical variation comes from the single-scanning fiber optic head design that ensures:

  • Each well receives the same amount of excitation light
  • Each well receives excitation light for the same length of time
  • Each well is the same distance from the detector
  • Eliminates the need for well-to-well signal correction by calibration or reference dyes. Uniformity of well-to-well measurements from the single-scanning fiber optic head design reduces the need for additional signal correction and calibration steps, streamlining your assay.

    Allows accurate quantification of low- to high-abundance targets. Use of a single photomultiplier tube (PMT) that provides linear detection over ten orders of magnitude ensures superior sensitivity and reproducibility.

    Increases flexibility. Four optical channels with user-selected filters that cover a broad range of excitation wavelengths, allowing use with most fluorescent dyes.

    Mx3005P QPCR System—Unmatched Flexibility

    The well-equipped Mx3005P QPCR System’s five-position user customizable filter wheel design allows you to choose which five filters are installed in your instrument from a list of eight filter sets spanning deep blue dyes to far red dyes.This flexibility enables:

  • Multiplex dye combinations with up to five targets per well
  • Custom excitation and emission filter pairs to accommodate the rapidly-expanding list of fluorescent dyes
  • Most fluorescent dye chemistries for FRET or other applications:
  • Ability to mismatch excitation and emission filters
  • Large Stokes shift dyes
  • Numerous applications your future research may need
  • Instrument Specifications

  • Excitation source: Quartz Tungsten Halogen lamp
  • Detector: 1 scanning photomultiplier tube (PMT)
  • Multiplexing capabilities: 5 targets in the same tube
  • Thermal system: solid-state, Peltier-based
  • Embedded computer safeguards data
  • Tube/plate formats: 200 µL , 96-well format, optimized for 25 µL sample size
  • Filters: Dual customizable filter wheels with four user-selected filters*
  • Dynamic Range: 10 orders of magnitude
  • Excitation Range: 350nm-750nm
  • Emission Range: 350nm-700nm
  • Temperature Uniformity: +/- .25°C at 72˚C
  • Temperature Ramp Rate: up to 2.5°C/second
  • Temperature Range: 25-99°C
  • Dimensions: 14" (35.6 cm) W x 20" (50.8 cm) D x 18" (45.7 cm) H, weight: 45 lbs (20.4 kg)
  • *Choice of filters includes: ALEXA Fluor 350 (350nm-440nm), FAM/SYBR® Green I (492nm-516nm), TET (517nm-538nm), HEX/JOE/VIC (535nm-555nm), Cy3 (545nm-568nm), TAMRA (556nm-580nm), ROX/Texas Red (585nm-610nm), Cy5 (635nm-665nm), ATTO (440nm-492nm), and HYB Probes (492nm-635nm).