pBlueScript II Vectors

The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites.

  • High copy number ColE1-based phagemid
  • Large and versatile polylinker in two orientations; f1 origin available in either (+) or (–) orientation
  • T3 and T7 promoters for in vitro transcription of RNA

 

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Catalog Kit Size Quantity Price  
R408 Intereference-Resistant Helper Phage

Stable and easy to grow helper phage (single-strand size ~4 Kb).

Certificate of Analysis
200252 1.0 ml $204.00
VCSM13 Interference-Resistant Helper Phage

Kanamycin-resistant helper phage (single-strand size ~6 Kb) capable high single-stranded phagemid yields. Derivative of M13KO7.

Certificate of Analysis
200251 1.0 ml $204.00
pBluescript II SK(+) Phagemid Kit

f1 origin in (+) orientation, Sac-->Kpn polylinker orientation, Contains: 20 µg pBluescript II SK(+) phagemid vector, Host Strain: XL1-Blue MRF'

Certificate of Analysis
212205 1 Kit $387.00
pBluescript II SK(-) Phagemid Kit

f1 origin in (-) orientation, Sac-->Kpn, polylinker orientation, Contains: 20 µg pBluescript II SK(-) phagemid vector, Host Strain: XL1-Blue MRF'

Certificate of Analysis
212206 1 Kit $387.00
pBluescript II KS(+) Phagemid Kit

f1 origin in (+) orientation, Kpn-->Sac polylinker orientation, Contains: 20 µg pBluescript II KS(+) phagemid vector, Host Strain: XL1-Blue MRF'

Certificate of Analysis
212207 1 Kit $387.00
pBluescript II KS(-) Phagemid Kit

f1 origin in (-) orientation, Kpn-->Sac polylinker orientation, Contains: 20 µg pBluescript II KS(-) phagemid vector, Host Strain: XL1-Blue MRF'

212208 1 Kit $387.00
pBluescript II XR Predigested Vector

XR vector is predigested with Xho I and EcoR I

Certificate of Analysis
212240 55 Rxn $393.00
pBluescript II RI Predigested Vector

RI vector is predigested with EcoR I

Certificate of Analysis
212250 55 Rxn $393.00