gDNA or Methylated cDNA

When DNA is methylated in a fashion unlike the bacterial host patterns, it is cleaved by the E. coli host restriction systems. Cleavage of DNA before host replication creates libraries that lack complete representation. Our MR (Minus Restriction) series of competent cells are deficient in all known E. coli K12 restriction systems and make it possible to clone methylated DNA, which can be very useful in epigenetic studies where DNA methylation, instead of changes in DNA sequence, may be associated with heritable changes in gene expression or cellular phenotype.

  • XL2-Blue MRF´ Ultracompetent Cells are high-efficiency derivatives of our XL1-Blue MRF´ Supercompetent Cells
  • XL1-Blue MRF’ Cells are deficient in all known restriction systems and are are endonuclease (endA), and recombination (recA) deficient
  • XL1-Blue MR Cells are restriction minus (McrA–, McrCB–, McrF–, Mrr, HsdR–) derivative of our XL1-Blue strain and is useful for cosmid-based cloning

 

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Catalog Kit Size Quantity Price  
XL1-Blue MRF' Kan Library Pack Competent Cells . Certificate of Analysis
200138 10 µg $390.00
XL2-Blue MRF’ Ultracompetent Cells

Restriction minus for cloning methylated DNA, highest cloning efficiency, > 5 x 109 transformants/µg

Certificate of Analysis

200151 10 x 0.1 mL $230.00
XL1-Blue MRF’ Electroporation-Competent Cells

Restriction minus for cloning methylated DNA, ≥ 1 x 1010 transformants/µg

Certificate of Analysis
200158 5 x 0.1 mL $214.00
XL1-Blue MR Supercompetent Cells

For use with cloning without the F' episome, ≥ 1 x 109 transformants/µg

Certificate of Analysis
200229 5 x 0.2 mL $198.00
XL1-Blue MRF’ Supercompetent Cells

Restriction minus for cloning methylated DNA, ≥ 1 x 109 transformants/µg

Certificate of Analysis
200230 5 x 0.2 mL $198.00
XL1-Blue MRF’ Kan Supercompetent Cells

Large aliquot size for scale-up library transformations, ≥1 x 109 transformants/µg

Certificate of Analysis
200248 5 x 0.2 mL $198.00
Routine Cloning Competent Cell Pack

We have competent cells to fulfill all your cloning needs. This economical competent cell pack lets you optimize your experimental design.

Certificate of Analysis
230248 1 Pack $109.00