Unstable Clones

Replicating eukaryotic DNA in prokaryotic cells can be problematic. Particular eukaryotic genes may contain inverted repeats or secondary structures, such as Z-DNA, that can be rearranged or deleted by E. coli DNA repair systems. The SURE competent cells are deficient in the E. coli genes involved in the rearrangement and deletion of DNA, thus improving cloning efficiencies of DNA containing irregular structures in prokaryotic cells. SURE cells are restriction minus (McrA-, McrCB-, McrF-, Mrr-, HsdR-) endonuclease (endA) deficient, and recombination (recB recJ) deficient.

  • Lack components of the pathways that catalyze the rearrangement and deletion of nonstandard secondary and tertiary structures
  • Allows for blue-white color screening

 

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Catalog Kit Size Quantity Price  
SURE 2 Supercompetent Cells

High-efficiency derivative for cloning DNA with secondary structures, > 1 x 109 transformants/µg

Certificate of Analysis
200152 10 x 0.1 mL $225.00
SURE Electroporation-Competent Cells

For cloning DNA with secondary structures, ≥1 x 1010 transformants/µg

Certificate of Analysis
200227 5 x 0.1 mL $232.00
SURE Competent Cells

For routine cloning of DNA with secondary structures, ≥5 x 108 transformants/µg

Certificate of Analysis
200238 5 x 0.2 mL $225.00
Difficult Cloning Competent Cell Pack

An economical way to solve your difficult cloning experiments. Try each strain with those clones that never seem to work.

Certificate of Analysis
230247 1 Pack $131.00