Brilliant HRM Ultra-Fast Loci Master Mix

Agilent provides Brilliant HRM Ultra-Fast Loci Master Mix for High Resolution Melt (HRM) analysis. The Master Mix combines a mutant Fast-Start Taq polymerase, optimized concentrations of MgCl2 and dNTPs, and EvaGreen, release-on-demand dye to enable researchers to mix-and-go faster and with total confidence - even for the most difficult genotypes. The optimized MgCl2 and dNTP concentrations reduce assay preparation time, while low surfactant levels minimize bubble trouble during pipetting. In addition, the 12-month storage at -20oC is more than double that of other HRM reagents.

  • Mix-and-Go. MgCl2 and dNTPs are included at optimized concentrations.
  • Better stability. Master Mix is stable after multiple freeze thaws, reducing wastage and increasing batch-to-batch reliability.
  • Fast-Start Taq. Proprietary, mutant Fast-Start Taq polymerase enzyme requires only three minutes for activation.
  • EvaGreen Release-on-Demand based dye. The dye is non-toxic and can be added at saturating concentrations - insuring minimal inhibition while preserving high sensitivity.
  • Platform independent. Master Mix can be used with the AriaMx Real-Time PCR System or any HRM-capable thermal cycler.
  • Try a sample of the Brilliant Ultra-Fast Loci Master Mix from the special offer below and learn more about Agilent’s HRM Solutions from this datasheet.
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    For Research Use Only. Not for use in diagnostic procedures.
    Product Catalog Kit Size Quantity Price  
    Brilliant HRM Ultra-Fast Loci Master Mix Sample

    15 reactions, 150µL Certificate of Analysis

    5190-7909 15 Rxn $54.37
    Brilliant HRM Ultra-Fast Loci Master Mix

    200 reactions, 2 mL

    Certificate of Analysis
    5190-7827 200 Rxn $206.00
    AriaMx HRM Calibration Kit

    1 x 96-well plate

    Certificate of Analysis
    5190-7702 96 Rxn $618.00
    Passive Reference Dye (Rox)

    1 mM, for controlling the final dye concentration. 10 vials @ 100 µL

    Certificate of Analysis
    600536 10 x 100 µL $262.00