Brilliant HRM Ultra-Fast Loci Master Mix

Agilent provides Brilliant HRM Ultra-Fast Loci Master Mix for High Resolution Melt (HRM) analysis. The Master Mix combines a mutant Fast-Start Taq polymerase, optimized concentrations of MgCl2 and dNTPs, and EvaGreen, release-on-demand dye to enable researchers to mix-and-go faster and with total confidence - even for the most difficult genotypes. The optimized MgCl2 and dNTP concentrations reduce assay preparation time, while low surfactant levels minimize bubble trouble during pipetting. In addition, the 12-month storage at -20oC is more than double that of other HRM reagents.

  • Mix-and-Go. MgCl2 and dNTPs are included at optimized concentrations.
  • Better stability. Master Mix is stable after multiple freeze thaws, reducing wastage and increasing batch-to-batch reliability.
  • Fast-Start Taq. Proprietary, mutant Fast-Start Taq polymerase enzyme requires only three minutes for activation.
  • EvaGreen Release-on-Demand based dye. The dye is non-toxic and can be added at saturating concentrations - insuring minimal inhibition while preserving high sensitivity.
  • Platform independent. Master Mix can be used with the AriaMx Real-Time PCR System or any HRM-capable thermal cycler.
  • Try a sample of the Brilliant Ultra-Fast Loci Master Mix from the special offer below and learn more about Agilent’s HRM Solutions from this datasheet.


    For Research Use Only. Not for use in diagnostic procedures.
    Product Catalog Kit Size Quantity Price  
    Brilliant HRM Ultra-Fast Loci Master Mix Sample

    15 reactions, 150µL Certificate of Analysis

    5190-7909 15 Rxn $54.37
    Brilliant HRM Ultra-Fast Loci Master Mix

    200 reactions, 2 mL

    Certificate of Analysis
    5190-7827 200 Rxn $206.00
    AriaMx HRM Calibration Kit

    1 x 96-well plate

    Certificate of Analysis
    5190-7702 96 Rxn $618.00
    Passive Reference Dye (Rox)

    1 mM, for controlling the final dye concentration. 10 vials @ 100 µL

    Certificate of Analysis
    600536 10 x 100 µL $262.00