T3 & T7 Polymerases

RUO

Bacteriophage T3 and T7 RNA polymerases are DNA-dependent RNA polymerases with high sequence specificity for T3 or T7 promoters. Both T3 and T7 RNA polymerases synthesize RNA 5' to 3' and incorporate ³⁵S, ³²P, and ³³P ribonucleotides. These enzymes are isolated from an overproducing recombinant E. coli clone.

For Research Use Only. Not for use in diagnostic procedures.

RNA Polymerases for PCR

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Features

Probes for nucleic acid hybridizations
Templates for in vitro translations
Substrates for RNA processing studies
Exon and intron mapping of genomic DNA

Specifications


Concentration	50 U/µL
Incubation Time	Incubate 30 minutes at 37 °C
Reaction Conditions	40mM Tris-HCl (pH 8.0), 8 mM MgCl2, 50 mM NaCl, 2 mM spermidine, 30 mM DTT, 400 µM of each rNTP, 1 µg DNA template and 10 units enzyme in a 25 µL volume. Incubate 30 minutes at 37°C
Storage Buffer	20 mM KPO4 (pH 7.7), 100 mM NaCl, 10 mM DTT, 1 mM EDTA, and 50 % glycerol (v/v)
Unit Definition	One unit will catalize the incorporation of 1.0 nmole of nucleoside triphosphate into an acid insoluble form in 60 minutes at 37°C

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