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Pfu DNA Ligase
Pfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosus hyperthermophilic marine archaebacterium that catalyzes linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45° to 80°C, with a temperature optimum near 70°C for nick-sealing reactions. It has higher ligation specificity and lower background than Tth DNA ligase.
- Highly thermostable, with half-life of over 60 minutes at 95°C
- Increases reliability of LCR by permitting higher melt temperatures
- Eliminates LCR template-predenaturation step, less enzyme required for LCR
For Research Use Only. Not for Use in Diagnostic Procedures.
| Product | Catalog | Kit Size | Quantity | Price | |
|---|---|---|---|---|---|
Pfu DNA Ligase, 400U
Kit contains: 400 U Pfu DNA Ligase 10X reaction buffer Certificate of Analysis |
600191 | 400 U | $346.00 |