Pfu DNA Ligase

Pfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosus hyperthermophilic marine archaebacterium that catalyzes linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45° to 80°C, with a temperature optimum near 70°C for nick-sealing reactions. It has higher ligation specificity and lower background than Tth DNA ligase.

  • Highly thermostable, with half-life of over 60 minutes at 95°C
  • Increases reliability of LCR by permitting higher melt temperatures
  • Eliminates LCR template-predenaturation step, less enzyme required for LCR

 

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Catalog Kit Size Quantity Price  
Pfu DNA Ligase, 400U

Kit contains: 400 U Pfu DNA Ligase 10X reaction buffer

Certificate of Analysis
600191 400 U $346.00