DNase I, RNase-free from bovine pancreas

Features

Produces no noticeable RNA degradation
One unit will completely digest 1 µg of DNA in 10 minutes at 37°C in a 25-µL reaction volume.
Incubation of 100-fold molar excess DNase I with RNA for one hour at 37°C produces no noticeable RNA degradation upon analysis by denaturing polyacrylamide gel electrophoresis.
Reaction conditions: 40 mM Tris-HCl (pH 7.5), 6 mM MgCl2, 2 mM CaCl2, 1 µg DNA and 1 unit enzyme in a 25 µL volume. Incubate 30 minutes at 37°C.
Storage Buffer: 10 mM Tris-HCl (pH 7.5), 10 mM CaCl2, 10 mM MgCl2 and 50 % glycerol (v/v). (Dilutions should be made in this buffer.)

Specifications


Concentration	10 U/µL
Incubation Time	Incubate 30 minutes at 37 °C
Physical Form	Premixed
Reaction Conditions	40 mM Tris-HCl (pH 7.5), 6 mM MgCl2, 2 mM CaCl2, 1 µg DNA and 1 unit enzyme in a 25 µL volume. Incubate 30 minutes at 37°C.
Storage Buffer	10 mM Tris-HCl (pH 7.5), 10 mM CaCl2, 10 mM MgCl2 and 50 % glycerol (v/v). (Dilutions should be made in this buffer.)
Substrate Type	RNA
Unit Definition	One unit will completely digest 1 µg of DNA in 10 minutes at 37°C in a 25 µL reaction volume.