NGS FFPE QC Kit

Formalin-fixed paraffin-embedded tissue represents a valuable sample source for molecular cancer research. These samples provide a contextual snapshot of the tissue at a specific time point and stage of disease but are highly challenging to analyze, especially using next generation sequencing. DNA derived from FFPE is oftentimes highly fragmented, cross-linked with protein and has a high proportion of single-stranded DNA. making it challenging for adaptor ligation and amplification, steps that are critical for successful preparation of sequencing libraries.

The Agilent NGS FFPE QC kit is a qPCR-based assay that enables functional DNA quality assessment of input DNA prior to preparation of next generation sequencing libraries. This kit enables assessment of the integrity of DNA as well as accurate quantitation of amplifiable template going into library preparation. Furthermore, optimizations to the SureSelectXT workflows based on the sample quality score are enabled.

 

  • Accurately qualify and quantify amplifiable DNA in challenging samples
  • Optimized low input library prep workflow for improved complexity and target coverage
  • Complete cancer research solutions from sample to data

 

 

For Research Use Only. Not for Use in Diagnostic Procedures

Product Catalog Kit Size Quantity Price  
Agilent NGS FFPE QC Kit

qPCR-based QC and Quant kit with reagents sufficient for processing 16 samples including reference and standard DNAs in triplicate

Certificate of Analysis
G9700A $330.00
Agilent NGS FFPE QC Kit

qPCR-based QC and Quant kit with reagents sufficient for processing 16 samples including reference and standard DNAs in triplicate

Certificate of Analysis
G9700B $1,681.00