SureGuide gRNA Synthesis Kit
RUO
The SureGuide gRNA Synthesis Kit provides quality-controlled reagents optimized for preparing CRISPR gRNA from a variety of user-selected templates. Part of Agilents integrated solution for in-vitro CRISPR/Cas research, this kit generates high-quality gRNA to combine with SureGuide Cas9 for cleaving DNA at the targeted sites with high accuracy. The SureGuide gRNA kit reduces your time from planning to experiment, and eliminates the cost of setting up your own CRISPR/Cas system.
For Research Use Only. Not for use in diagnostic procedures.
- CRISPR Kits
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This product is not available for purchase by the general public.
Product Details
Features
- Kit components are formulated for a protocol to synthesize and purify gRNAs in under 2 hours, saving you time.
- This extensively optimized and validated synthetic system frees you up to troubleshoot your application not our reagents.
- Enzymes and reagents are optimized for use in conjunction with SureGuide Cas9 Nuclease for worry-free performance.
- The kit is suited for gRNA transcription from a variety of DNA template designs to provide flexibility.
- The kit includes Control Template DNA to confirm the success of your reactions
Specifications
Control SgRNA |
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Includes Buffer |
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Includes IVT Enzyme |
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Number of Reactions |
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Literature
- Data Sheets
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SureGuide Cas9 Programmable Nuclease Kit_5991-5149EN
The CRISPR/Cas in vitro system is a complete and ready-to-use kit for performing unrestricted in vitro cloning of large DNA fragments without the limitation of common RE's
- Data Sheets
- English
- 02 Sep 2014
- 748.47 KB
SureGuide gRNA Synthesis Kit Datasheet 5991-5148EN
The SureGuide gRNA synthesis kit is designed for the rapid and simple preparation of gRNAs to be used in conjunction with the SureGuide Cas9 system (part no 5190-7714).
- Data Sheets
- English
- 29 Aug 2014
- 744.08 KB
- Flyers
Support
- User Manuals
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SureGuide gRNA Synthesis Kit Protocol
SureGuide gRNA Synthesis Kit Protocol
- User Manuals
- English
- 07 Apr 2015
- 688.02 KB
Featured References
- Publications
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- Tatiana V. Egorova, Evgenia D. Zotova, Denis A. Reshetov, Anna V. Polikarpova, Svetlana G. Vassilieva, Dmitry V. Vlodavets4, Alexey A. Gavrilov5, Sergey V. Ulianov, Vladimir L. Buchman and Alexei V. Deykin. CRISPR/Cas9-generated mouse model of Duchenne muscular dystrophy recapitulating a newly identified large 430 kb deletion in the human DMD gene. Disease Models & Mechanisms (2019) 12, dmm037655. doi:10.1242/dmm.037655 Learn More
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