QuikChange Primer Design

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The QuikChange® Primer Design Program assists you in designing mutagenic primers for your QuikChange site-directed mutagenesis experiments. This user-friendly program provides you the appropriate primer sequences with the optimal melting temperature and supports all of our QuikChange® site-directed mutagenesis kits. Features include:

  • Ability to design primers for point mutations, deletions and insertions
  • Primer suggestions for multi-site mutagenesis and saturation mutagenesis
  • Translation of imported DNA sequence to amino acid sequence
  • Generation of mutagenic primers with appropriate melting temperature and free energy

Disclaimer: This program is provided at no charge to our customers. Due to variation in certain sequences some designs may not result in the appropriate primer sequence for mutagenesis. It is highly recommended that all Tm calculations and sequences be verified prior to use.