QPCR Standard Curve Slope to Efficiency Calculator
Calculator to convert the slope produced by a QPCR standard curve to % efficiency. It also gives the exponent and amplification
This calculator uses the slope produced by a QPCR standard curve to calculate the efficiency of the PCR reaction. Slopes between -3.1 and -3.6 giving reaction efficiencies between 90 and 110% are typically acceptable.
The formula for this calculation is Efficiency = -1+10(-1/slope)